Genomics of Clubroot Disease Development in Canola and Development of in planta RNAi to Impart Novel Resistance

Researcher: Peta Bonham-Smith and Yangdou Wei, University of Saskatchewan; Gary Peng and Bruce Gossen, Agriculture and Agri-Food Canada, Saskatoon SK

Project Code: CGDP.SCDC.ADF 2010-98

Final Report: February 2016

Summary: Clubroot, resulting from an infection by Plasmodiophora brassicae is a devastating
disease of canola. Researchers initiated a five-year study in 2010 to investigate the
cellular and molecular mechanisms of P. brassicae infection, and to develop molecular
markers and tools for utilizing pathogen protein targets for improved resistance to
clubroot disease in canola. As a result of the project, a cDNA library was constructed,
from which over 2000 P. brassicae sequences were isolated. A transcriptome analysis
of infected roots was also established and will be used to identify and develop
molecular markers for commercial canola resistance breeding programs. The results
from this work will be of use to the clubroot research community in the utilisation of
pathogen protein targets for improved resistance to clubroot disease in canola.

Click the links below to download a short version of the report, the full detailed long version of the report, or the related Canola Digest PDF.

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